ID Number		1442
Study Type		In Vitro
Model		High voltage electrical stimulation of cells and analysis of molecule uptake (electroporation)
Details		Yeast and erythrocytes were exposed to high voltage electric currents and analyzed for uptake of the 70 kDa molecule FITC-dextran and the 660 Da molecule propidium iodide by flow cytometry (via green or red fluorescence, respectively). The authors report that 20% of erythrocytes and 75% of yeast can take up the first test molecule within 5 minutes of a pulse. The uptake of the smaller propidium iodide molecule following initial uptake of FITC-dextran provides a measure of membrane recovery
Findings		Effects (only at thermal levels)
Status		Completed With Publication
Principal Investigator		Harvard-MIT Health Sciences and Technology, USA
Funding Agency		NIH, USA
Country		UNITED STATES
References		Smith, KC et al. Biophys J, (2008) 95:1547-1563 Weaver, JC J Radio Sci, (1995) 30:205-221 Bliss, JG et al. Bioelectrochem Bioenerg, (1989) 20:57-71 Weaver, JC et al. FEBS Letters, (1988) 229:30-34
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