ID Number		1556
Study Type		Engineering & Physics
Model		Exposure Systems - Cell Culture (catch all)
Details		Exposure Systems - Cell Culture (catch all) AUTHORS' ABSTRACT: Collin et al. 2016 (IEEE #6346): An in vitro exposure setup developed to expose suspended cells in Petri dishes on a large frequency band up to a few gigahertz was studied. This system was based on far-field exposure with a horn antenna in an anechoic chamber. A specific incubator was designed with a water-cooling system for constant temperature inside the setup. Four exposed Petri dishes presented a similar specific absorption rate (SAR) distribution with a whole-volume SAR of 0.56 ±0.40 W/kg/Winc at 2.45 GHz. The incubator was equipped with a stirring mechanism allowing an homogenous distribution of temperature in the biological solution. The SAR was assessed over the 1.5-3 GHz frequency range. Using the temperature control and stirring, an increase of 0.4°C in temperature was observed after 2 h exposure at 2.45 GHz, 22 W incident power. This dosimetric study demonstrated the ability of this system to expose cells in suspension at different frequencies in a controlled environment. AUTHOR'S ABSTRACT: Oster et al. 2016 (IEEE #6382): Neuronal networks in vitro are considered one of the most promising targets of research to assess potential electromagnetic field induced effects on neuronal functionality. A few exposure studies revealed there is currently no evidence of any adverse health effects caused by weak electromagnetic fields. Nevertheless, some published results are inconsistent. Particularly, doubts have been raised regarding possible athermal biological effects in the young brain during neuronal development. Therefore, we developed and characterized a flexible experimental setup based on a transverse electromagnetic waveguide, allowing controlled, reproducible exposure of developing neuronal networks in vitro. Measurement of S-parameters confirmed very good performance of the Stripline in the band of 800-1000 MHz. Simulations suggested a flexible positioning of cell culture dishes throughout a large exposure area, as specific absorption rate values were quite independent of their position (361.7 ± 11.4 mW/kg) at 1 W, 900 MHz. During exposure, thermal drift inside cellular medium did not exceed 0.1 K. Embryonic rat cortical neurons were cultivated on microelectrode array chips to non-invasively assess electrophysiological properties of electrogenic networks. Measurements were taken for several weeks, which attest to the experimental setup being a reliable system for long-term studies on developing neuronal tissue. AUTHORS' ABSTRACT: Garc1a-Fernandez, Veyret et al. 2016 (IEEE #6538): In this paper, the dosimetric characterization of an EMF exposure setup compatible with real-time impedance measurements of adherent biological cells is proposed. The EMF are directly delivered to the 16-well format plate used by the commercial xCELLigence apparatus. Experiments and numerical simulations were carried out for the dosimetric analysis. The reflection coefficient was less than 10 dB up to 180 MHz and this exposure system can be matched at higher frequencies up to 900 and 1800 MHz. The specific absorption rate (SAR) distribution within the wells containing the biological medium was calculated by numerical finite-difference time domain simulations and results were verified by temperature measurements at 13.56 MHz. Numerical SAR values were obtained at the microelectrode level where the biological cells were exposed to EMF including 13.56, 900, and 1800 MHz. At 13.56 MHz, the SAR values, within the cell layer and the 270-¼L volume of medium, are 1.9e3 and 3.5 W/kg/incident mW, respectively.
Findings
Status		Completed With Publication
Principal Investigator
Funding Agency		?????
Country		UNITED STATES
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