ID Number		1939
Study Type		In Vivo
Model		50 Hz ELF exposure to laboratory animals and analysis of oxidative stress endpoints in liver tissue,
Details		Guinea pigs were exposed to 50 Hz (ELF) using a pair of copper plates set 18cm apart and yielding an E-field strength of 12 KV/m. Exposures were performed either with or without injection of the anti-oxidant N-acityl-L-cysteine (NAC - given 30 minutes prior to exposure). The authors report no oxidative damage with exposure, but do report decreased levels of NAC and hydroxyproline in liver homogenates of exposed animals suggesting decreased collagen levels that the authors speculate may be the result of oxidized collagen proteins. AUTHORS' ABSTRACT: Guler et al. 2009 (IEEE #5771): In order to test whether antioxidants have beneficiary effects on electric field induced damage, we determined the pulmonary levels of heme oxygenase-1 (HO-1), protein carbonyl content (PCO), malondialdehyde (MDA), nitric oxide (NO) and hydroxyproline (HP) under extremely low frequency (ELF) electric (E) field exposure (50 Hz, 12 kV/m, 7 days/for 8 h/day). While PCO levels significantly increased (p<0.05), insignificant changes (p>0.05) were observed in HO-1, MDA, NO and HP levels for electric field exposure groups compared to the control group. We have not observed any significant change in these parameters on the electric field group compared to the group where NAC and EGCG were separately applied along with electric field. However, during our previous studies, we have concluded that NAC and EGCG are potent antioxidants and we believe that new studies should be established by way of setting up different experimental conditions. AUTHORS' ABSTRACT: Guler, Seyhan et al. 2008 (IEEE #6084): PURPOSE: To investigate the effects of 12 kV/m electric (E) field sourced by power lines on oxidative and nitrosative stress, and antioxidant status. Furthermore, the study aimed to examine the protective effects of N-Acetyl-L-cysteine (NAC) and epigallocatechin-gallate (EGCG) in the liver tissues of guinea pigs against the possible detriments of electromagnetic field exposure. MATERIALS AND METHODS: Guinea pigs were exposed to 50 Hz 12 kV/m E-field. NAC and EGCG were administered intraperitoneally. Malonedialdehyde (MDA), a product of lipid peroxidation (LPO), and nitric oxide derivatives (nitrate (NO(3)), nitrite (NO(2)), total level of nitric oxide (NO(x)) were estimated as biomarkers of oxidative and nitrosative stress, respectively. Superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and myeloperoxidase (MPO) were evaluated as endogenous antioxidant enzymes in liver tissues of the guinea pigs. RESULTS: The results of our study indicated a significant increase in the levels of oxidant products (MDA, NO(3), NO(2), NO(x)), and a significant decrease in antioxidant enzyme (SOD, GSH-Px and MPO) activities. We also found that the individual or plus application of NAC and EGCG resulted in the reduction of oxidative stress prior to E field application. CONCLUSION: To conclude, extremely low frequency (ELF) electric field has potential harmful effects on the living organisms by enhancing the free radical production. NAC and EGCG might have hepatoprotective effects in ELF-E field induced oxidative and nitrosative stress.
Findings		Effects
Status		Completed With Publication
Principal Investigator		Gazi University, Ankara, Turkey - arintomruk@gmail.com
Funding Agency		EU, Gazi University Research Foundation
Country		TURKEY
References		Guler, G et al. Gen Physiol Biophys, (2009) 28:47-55 Guler , G et al. Sci Total Environ., (2009) 407:1326-1332 Guler, G et al. Int J Radiat Biol., (2008) 84:669-680
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