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EMF Study
(Database last updated on Mar 27, 2024)
ID Number |
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2506 |
Study Type |
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In Vitro |
Model |
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Study of biological effects (cell proliferation, cell death, cell viability, and DNA synthesis rates) of ELF-MF (1 mT, 60 Hz, 4 or 16 h) on MCF10A, MCF7, Jurkat, and NIH3T3 cell lines. |
Details |
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AUTHORS' ABSTRACT: Lee et al. 2015 (IEEE #6096): Owing to concerns regarding possible effects of extremely low frequency magnetic fields (ELF-MF)
on human health, many studies have been conducted to elucidate whether ELF-MF can induce
modifications in biological processes. Despite this, controversies regarding effects of ELF-MF are
still rife. In this study, we investigated biological effects of ELF-MF on MCF10A, MCF7, Jurkat,
and NIH3T3 cell lines. ELF-MF with a magnetic flux density of 1mT at 60Hz was employed to
stimulate cells for 4 or 16 h, after which the effects of ELF-MF on cell proliferation, cell death, cell
viability, and DNA synthesis rates were assessed. Whereas Jurkat and NIH3T3 cells showed no
consistent variation in cell number, cell viability, and DNA synthesis rate, MCF10A and MCF7
cells showed consistent and significant decreases in cell number, cell viability, and DNA synthesis
rates. However, there was no effect of ELF-MF on cell death in any of tested cell lines. Next, to
investigate the effect of ELF-MF on gene expression, we exposed MCF7 cells to 2mT at 60Hz for
16 h and examined transcriptional responses by using gene expression array. We found a gene,
PMAIP1, that exhibited statistically significant variation using two-fold cut-off criteria and certified
its expression change by using semi-quantitative and quantitative reverse transcription polymerase
chain reaction. From these results, we concluded that ELF-MF could induce the delay of cell cycle
progression in MCF7 and MCF10A cells in a cell context-specific manner and could up-regulate
PMAIP1 in MCF7 cells. |
Findings |
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Effects |
Status |
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Completed With Publication |
Principal Investigator |
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Inha University, Incheon, Korea
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Funding Agency |
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?????
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Country |
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KOREA, REPUBLIC OF |
References |
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Lee, HC et al. Bioelectromagnetics., (2015) 36:506-516
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Comments |
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