ID Number		630
Study Type		In Vitro
Model		1800 MHz(GSM) exposure to mouse primary astrocytes + pig brain capillary endothelial cells and analysis of sucrose permeability (in vitro BBB model)
Details		Astrocytes from neonatal rat brain and porcine brain capillary endothelial cells were co-cultured to simulate brain and examine the effects of RF on blood brain barrier. Cultures were exposed to 1.8 GHz (GSM) RF for 4 days at an average SAR of 0.3 W/kg (0.46 W/kg peak) in a rectangular waveguide. During the exposure period, permeability was examined at various time points during the 4 day exposure period by morphological analysis, electron microscopy (close apposition of cells, lack of intracellular clefts), immunocytochemistry (zona occludens protein leakage), and 14C-sucrose leakage. The authors report that exposure significantly increased the permeability of 14C-sucrose across the endothelial cell boundry (using data from 16 exposed and 9 control cultures; p < 0.001) Infra red thermometry of the culture samples was used to verify that the temperature increases due to exposure were not significant.
Findings		Effects
Status		Completed With Publication
Principal Investigator		University of Bordeaux, France - b.veyret@piom.u-bordeaux.fr
Funding Agency		CoMoBio, France
Country		GERMANY
References		Schirmacher, A et al. Bioelectromagnetics, (2000) 21:338-345
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