ID Number		811
Study Type		In Vitro
Model		16, 50 Hz exposure to isolated pineal glands of Djungarian hamsters and analysis of melatonin level
Details		Isolated pineal glands of Djungarian hamsters (Phodopus sungorus) were exposed to magnetic fields (16.67 or 50 Hz at 86 microTesla)for 8 hr. In each experiment, 12 exposed and 12 sham-exposed pineal glands were placed individually in glass chambers which were surrounded by identical coils. After 3.25 hours of adaptation, melatonin production was stimulated by isoproterenol (10-7 M) for 30 min. Eluted fractions were collected, and melatonin concentrations were measured by radioimmunoassay. Statistical analyses for each frequency showed significant suppressive effects at 162/3 Hz (P<0.01) and 50 Hz (P<0.001). It is concluded that the suppressive effects of magnetic fields on the synthesis of melatonin are a result of primary mechanisms at the level of the pineal gland.
Findings		Effects
Status		Completed With Publication
Principal Investigator		International University Bremen, Germany - a.lerchl@iu-bremen.de
Funding Agency		FGF, Germany
Country		GERMANY
References		Brendel, H et al. J Pineal Res., (2000) 29:228-233
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